New Delhi, Feb 8 A team of scientists has developed a new technology platform for fluorometric detection of pathogens such as viruses by measurement of fluorescent light emitted and the potential of the new technology has been demonstrated for the detection of SARS-CoV-2.
This technology platform can also be used to detect other DNA/RNA pathogens such as HIV, influenza, HCV, Zika, Ebola, bacteria, and other mutating/evolving pathogens.
The ongoing Covid-19 pandemic caused by SARS-CoV-2 continues to inflict catastrophic effects on all aspects of our lives, ample proof of how viruses are a major global threat to human health. The unprecedented transmission rate of RNA virus has necessitated the rapid and accurate diagnosis to facilitate contact tracing (prevent spreading) and to provide timely treatment.
Scientists from Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), an autonomous institute of the Department of Science & Technology, Government of India, along with scientists from Indian Institute of Science (IISc), have demonstrated a noncanonical nucleic acid-based G-quadruplex (GQ) topology targeted reliable conformational polymorphism (GQ-RCP) platform to diagnose Covid-19 clinical samples.
"The present work demonstrated the first GQ-targeted diagnostic platform for SARS-CoV-2 in clinical samples, based on a novel platform GQ-RCP. This molecular detection platform can be integrated into field-deployable isothermal amplification assays with more reliability and sequence specificity," said a release from the Ministry of Science & Technology.
Authored by Sumon Pratihar, Ragini Agrawal, Virender Kumar Pal, Amit Singh, and Thimmaiah Govindaraju (corresponding author), this work has been published recently in the journal 'ACS Sensors' and the team has also filed a patent for the novel technology.
The platform lays greater emphasis on deciphering and systematic characterisation of a unique set of interactions in nucleic acids to attain stable and reliable noncanonical DNA/RNA targets. The RCP-based target validation is a general and modular approach for the development of noncanonical nucleic acid-targeted diagnostic platforms for diverse pathogens, including bacteria and DNA/RNA viruses.
RT-q-PCR has been the gold standard for accurate detection of SARS-CoV-2 (Covid-19). Among the recent innovations on nucleic acid-targeted diagnosis of SARS-CoV-2, the techniques such as RT RPA and RT-LAMP use general-purpose DNA sensing probes. This increases the propensity of false-positive results arising out of unbiased detection of nonspecific amplification products. Recognizing unique DNA secondary conformations can be a promising solution to achieve reliable readouts.
The team has identified and characterised a unique G-quadruplex-based target derived from the 30 kb (kilobytes) genomic landscape of SARS-CoV-2 for specific detection of SARS-CoV-2. Unlike the other reliable diagnostic assays where the existing fundamental concepts have been repurposed, this work presents a completely novel strategy to target a unique, unconventional structure specific to the SARS-CoV-2 sequence using small molecule fluorophores (microscopic molecules), the release said.
The team developed GQ topology targeted detection of SARS-CoV-2 derived DNA obtained after reverse transcription and amplification from genomic RNA in clinical samples. The GQ targeted detection was achieved by pH-triggered facile transformation of the amplified double-stranded DNA into stable GQs, which forms the target for detection with remarkable selectivity using a designed fluorescent dye which is a benzobisthiazole-based target-specific turn on called BTMA.
"Thus, this study demonstrates a reliable strategy for fluorogenic organic molecule-based GQ-RCP platform to diagnose Covid-19 clinical samples and is the first practical demonstration of it," it added.
"We have demonstrated rational tailoring of molecular probes to achieve unambiguous target recognition and increase the reliability of detection, at a shorter time without the requirement of an expensive RT-q-PCR instrument. This RCP-based platform is very general and can be easily adopted for the detection of various DNA/RNA based pathogens including bacteria and viruses such as HIV, Influenza, HCV, etc.," said Govindaraju.
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