Soon after the House reassembled at 2 p.m., BJP Member of Parliament Meenakshi Lekhi, who was on the chair, continued the proceedings and papers were laid on the table.
Meanwhile, Congress members again trooped near the Speaker's podium raising slogans against the government and displaying placards.
Amid the din, Lekhi continued the House proceedings and the concerned minister laid papers listed for the business during the day.
As Congress MPs continued their protest, Lekhi adjourned the House till 2 p.m. on Tuesday.
She also announced that all MPs will attend the programme on the occasion of the Constitution Day on Tuesday in Parliament at 11 a.m. in which President Ram Nath Kovind, Vice President M. Venkaiah Naidu, Prime Minister Narendra Modi and several Union Ministers and leaders will be present.
Earlier in the day, the Lok Sabha was adjourned twice soon after the House met following a brief clash between two Congress MPs and the marshals during Question Hour as well as a protest led by Congress over Maharashtra developments.
Speaker Om Birla adjourned the House till 2 p.m. soon after it met for the second time at 12 noon.
Earlier in the day, Birla ordered marshals to take two Congress members - Hibi Eden and T N Prathapan - who were holding a banner with 'stop murder of democracy slogan' - out of the House. Eden and Prathapan were holding the black banner which sported the slogan 'stop murder of democracy' while placards had messages like 'save Constitution' and 'save democracy'.
However, the two MPS were joined by other Congress members in resisting their eviction from the House and were seen jostling with marshals, prompting the Speaker to adjourn the House till 2 p.m.
When the House met and Question Hour began, the Congress members trooped near Speaker's podium.
Former Congress chief Rahul Gandhi said he was denied to raise question when Speaker called his name during the Question Hour. Rahul Gandhi said, "I had come here to ask a question. But I would not ask as democracy has been murdered in Maharashtra."
The Nationalist Congress Party (NCP), however, was missing from the Lok Sabha while the National Conference joined Congress protest in the House.
The Congress MPs, including party interim Chief Sonia Gandhi on Monday morning staged protest near Mahatma Gandhi statue in Parliament over Maharashtra developments.
The Congress has been protesting against the manner in which Devendra Fadnavis took oath as Maharashtra Chief Minister and Ajit Pawar, nephew of NCP supremo Sharad Pawar as his deputy on Saturday morning.
The Congress had earlier said it will stall proceedings in both the Lok Sabha and the Rajya Sabha over the manner in which Fadnavis was sworn in as Maharashtra Chief Minister in an early morning ceremony on Saturday.
Earlier, the Congress parliamentary strategy group, which includes Ghulam Nabi Azad, Adhir Ranjan Chowdhury and K.C. Venugopal, met at Sonia Gandhi's residence 10 Janpath in New Delhi.
( With inputs from IANS )