A team of researchers from Rice University in collaboration with Baylor College of Medicine developed a new device that mimics intestines, giving them a mechcal model to track the real-time growth of bacterial infections.
The new study demonstrates a lab tool that simplifies simulations of the human intestine, making it more practical to find treatments for diseases like infectious diarrhea.
The team led by bioengineer Jane Grande-Allen of Rice's Brown School of Engineering developed transparent millifluidic perfusion cassettes (mPCs) that are easy to fabricate and operate and compatible with common microscopic and biochemical analysis.
The cassettes allow even non-bioengineers to perform the kind of studies typically done with a 96-well petri dish, with the added benefit of fluid flow overseeded epithelial cells infected with bacteria. The cassettes also have micro-scale ports for input and output, allowing not only for fluid flow but also for a sampling of the environment.
Replicating the complicated human gut is a challenge for researchers, said co-author Anthony Maresso, an associate professor of molecular virology and microbiology at Baylor. "This one was designed to be easy to use by scientists with less engineering know-how. The hope is it will lower barriers between engineers and medical researchers," he said.
Molds of the devices, about the size of standard 96-well plates, were created with a 3D printer and used to form the clear polymer cassettes. The mPCs were seeded with human intestinal enteroids (HIEs), cultures that contain the major cell types native to the intestinal epithelium. When fluid containing bacteria flowed through, it formed a biofilm on the cells, a phenomenon not seen in static platforms.
Grande-Allen said making a microfluidic system that doesn't leak can be technically challenging. "In this case, we needed to mimic the rate of fluid throughout the intestine that, especially compared to blood, is really slow," she said.
Researchers can easily evaluate how well bacteria adhere to and infect the cells, both visually and by sampling through the ports on either end. Static plates allow bacteria to overgrow the cells and limit experiments to a few hours, but the slow flow through mPCs allows extended observation and more realistic results, Grande-Allen said.
Testing the device led the researchers to find the first direct evidence that aggregative adherence fimbriae, sticky appendages found on most infectious enteroaggregative Escherichia coli (EAEC), are necessary for the bacteria to form a biofilm.
"Our intention was to slowly flush the bacterial toxins to keep them from harming the cells. That allowed more cells to survive so we could watch the process of infection," Grande-Allen said.
The devices also keep HIE cultures alive by replenishing oxygen and nutrients in the mPC chamber, allowing for better evaluation of realistic interactions between the cells and invading pathogens, researchers told.
According to Grande-Allen, mPCs will facilitate research on many such interactions. "This will allow the systematic examination of a lot of different combinations, good and bad, and how flow changes the dynamics of this environment," she said.
( With inputs from ANI )
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